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Research Scientist – Biology at Repare Therapeutics
Montreal, CA

Repare Therapeutics is a 2017 “Fierce 15” start-up backed by tier-one strategic and institutional biotechnology investors (http://www.reparerx.com). Repare develops novel precision oncology drugs that target specific vulnerabilities of tumor cells. This is accomplished by combining proprietary CRISPR‐enabled genome-wide synthetic lethal (SL) screens for target discovery and patient selection biomarkers with high‐resolution protein crystallography, computational biology and clinical informatics. Founded by leading experts at the forefront of DNA damage and repair, cancer biology, and high-throughput CRISPR screening technologies, Repare has assembled a seasoned team of industry veterans and a world-class Advisory Board.

 

We now have an outstanding opportunity available for a motivated M.Sc. or Ph.D.-level research scientist who would like to get in early and grow with the company. The successful candidate will have experience in at least one of the following research areas: DNA repair, epigenetics, cancer biology, synthetic lethality, and/or genome engineering. The candidate will become a vital member of our SL platform team where he/she will contribute both experimentally and intellectually to design and execute new SL campaigns and to generate a pool of completely validated drug targets to advance our portfolio. A critical component of the job will be the close collaboration with internal drug discovery teams and external investigators.

 

RESPONSIBILITIES

  • Utilize CRISPR/Cas9 genome editing and other cell biology tools to generate and characterize cell line models for SL screens, target validation, and mechanism of action studies
  • Design and execute CRISPR-enabled genome-wide SL screens
  • Fully characterize cancer cell line panels: expanding, banking, and confirming loss of function or gain of function gene mutations by sequencing, qPCR, immunoblotting, and/or functional assays
  • Apply skill set to identify and fully validate new SL targets
  • Plan and execute experiments to uncover mechanisms underlying SL interactions
  • Facilitate the development of computational biology tools by working closely with our bioinformatics team

QUALIFICATIONS

  • M.Sc. or Ph.D. in Molecular Genetics, Cancer Biology, Cell and Molecular Biology, or related fields
  • At least 2 years experience in mammalian cell culture
  • Understanding or hands-on experience in genome editing techniques (CRISPR, TALENs, ZFNs, etc.) to generate gene knockouts, point mutants, and knock-in cell lines
  • Experience in functional genomics (especially RNAi or CRISPR screens) is highly desired
  • Expertise in cell and molecular biology techniques needed (proliferation assays, microscopy, cytogenetics, PCR, immunoblotting, etc.)
  • Experience in lentiviral or retroviral packaging and cell line transductions would be an asset
  • Excellent communication skills. Ability to communicate in an open, clear, timely and consistent manner
  • Outstanding organizational skills (lab book, experimental design, reagent cataloging, etc.)
  • Ability to handle multiple projects and utilize judgement to prioritize tasks
  • Ability to work as a team member in a dynamic, fast-paced biotech environment where you can make a clear impact
  • Experience in pre-clinical drug discovery is an asset